Antioxidant and Antiproliferative Activities of Myrtus communis L. Essential Oils from Different Algerian Regions

Hennia, A., Nemmiche, S., Guerreiro, A., Faleiro, M. L., Antunes, M. D., Aazza, S., Miguel, M. G. (2019)


To evaluate the antioxidant activity of fourteen essential oils (EOs) isolated from myrtle (Myrtus communis L.) collected at different regions of Algeria, seven different assays were carried out: ability for scavenging ABTS (2,2-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), HO (hydroxyl), NO (nitric oxide) free radicals; preventing lipid peroxidation through the thiobarbituric reactive species method, for reducing ferric ions to ferrous ions and chelating activity. The antiproliferative capacity of the EOs on Caco-2 cells were also evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The results showed that the essential oils were mainly constituted by α-pinene, 1,8-cineole, limonene and linalool. The EOs at different concentrations showed moderate to poor antioxidant activity. The sample Mc_Lf_06 was the single one that presented higher antioxidant activity across the four assays (prevention of lipid peroxidation, ability for scavenging the ABTS, NO and hydroxyl radicals). The EOs Mc_Lf_06 and Mc_Lf_10 showed the best antiproliferative activity but only achieving 20 % inhibition after 96 h of incubation for all concentrations tested. Intriguing the sample Mc_Lf_07 that stimulated the proliferation of Caco-2 cells, particularly at lower concentrations (0.1 and 0.2 mg/mL).

Keywords: Free radicals; lipid peroxidation; Caco-2; myrtle; Algeria

Consulte este Artigo Científico aqui.

Últimas notíciaspublicaçõescomunicações